Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Thirty–two strains were isolated from 15 soils sampled in central Iran, by using and comparing three different methods. The screening of soil samples by means of soil paste– plate method combined with isolation on mannitol agar proved to be the best strategy in terms of reliability and selectivity. These test isolates were biochemically characterized. These isolates were screened in vitro and identified by using BIBI(Bioinformatics Bacterial Identification Tool). BIBI was designed to automate DNA sequence analysis for bacterial identification in the different fields. BIBI relies on the use of BLAST and CLUSTAL W programs applied to different subsets of sequences extracted from GenBank. These sequences are filtered and stored in a new database, which is adapted to bacterial identification.
Mazinani, Z., Aminafshar, M., Asgharzadeh, A., & Chamani, M. (2012). Different Methods for Isolation and Preliminary Identification of Azotobacter. International Journal of Agricultural Science and Research, 3(Issue 1), 1-80.
MLA
Z. Mazinani; M. Aminafshar; A. Asgharzadeh; M. Chamani. "Different Methods for Isolation and Preliminary Identification of Azotobacter". International Journal of Agricultural Science and Research, 3, Issue 1, 2012, 1-80.
HARVARD
Mazinani, Z., Aminafshar, M., Asgharzadeh, A., Chamani, M. (2012). 'Different Methods for Isolation and Preliminary Identification of Azotobacter', International Journal of Agricultural Science and Research, 3(Issue 1), pp. 1-80.
VANCOUVER
Mazinani, Z., Aminafshar, M., Asgharzadeh, A., Chamani, M. Different Methods for Isolation and Preliminary Identification of Azotobacter. International Journal of Agricultural Science and Research, 2012; 3(Issue 1): 1-80.
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